Activity Assay:Article Title: Engineering exosomes with iRGD for targeted RNAi therapy against pancreatic cancer mediated by long non-coding RNA PLBD1-AS1.
Article Snippet: .. These data suggest that exosomes can effectively deliver siPLBD1-AS1 into target cells and exert biological effects, with iRGD further improving cellular uptake and enhancing their anticancer activity. analysis of α-SMA, VIM, FAP, and β-actin in PSCs after treatment with tumor cell-derived exosomes (n = 3). (C) ELISA of IL-6, MCP-1, and FN levels in the supernatant of PSCs incubated with tumor cell-derived exosomes (n = 3). (E) ECAR and OCR measurements of PSCs following exosome treatment (n = 3). (F) Intracellular and extracellular lactate levels in PSCs after exosome incubation (n = 3). (G) ALDOA enzyme activity in PSCs treated with tumor cell-derived exosomes (n = 3). (H) Western blot analysis of α-SMA, VIM, FAP, and GAPDH in PSCs incubated with exosomes under low glucose, 2-DG, or ALDOA knockdown conditions (n = 3). (I) ELISA of IL-6, MCP-1, and FN in the supernatant of PSCs treated with exosomes under low glucose, 2-DG, or ALDOA knockdown (n = 3). ..
Enzyme-linked Immunosorbent Assay:Article Title: Engineering exosomes with iRGD for targeted RNAi therapy against pancreatic cancer mediated by long non-coding RNA PLBD1-AS1.
Article Snippet: .. These data suggest that exosomes can effectively deliver siPLBD1-AS1 into target cells and exert biological effects, with iRGD further improving cellular uptake and enhancing their anticancer activity. analysis of α-SMA, VIM, FAP, and β-actin in PSCs after treatment with tumor cell-derived exosomes (n = 3). (C) ELISA of IL-6, MCP-1, and FN levels in the supernatant of PSCs incubated with tumor cell-derived exosomes (n = 3). (E) ECAR and OCR measurements of PSCs following exosome treatment (n = 3). (F) Intracellular and extracellular lactate levels in PSCs after exosome incubation (n = 3). (G) ALDOA enzyme activity in PSCs treated with tumor cell-derived exosomes (n = 3). (H) Western blot analysis of α-SMA, VIM, FAP, and GAPDH in PSCs incubated with exosomes under low glucose, 2-DG, or ALDOA knockdown conditions (n = 3). (I) ELISA of IL-6, MCP-1, and FN in the supernatant of PSCs treated with exosomes under low glucose, 2-DG, or ALDOA knockdown (n = 3). ..
Incubation:Article Title: Engineering exosomes with iRGD for targeted RNAi therapy against pancreatic cancer mediated by long non-coding RNA PLBD1-AS1.
Article Snippet: .. These data suggest that exosomes can effectively deliver siPLBD1-AS1 into target cells and exert biological effects, with iRGD further improving cellular uptake and enhancing their anticancer activity. analysis of α-SMA, VIM, FAP, and β-actin in PSCs after treatment with tumor cell-derived exosomes (n = 3). (C) ELISA of IL-6, MCP-1, and FN levels in the supernatant of PSCs incubated with tumor cell-derived exosomes (n = 3). (E) ECAR and OCR measurements of PSCs following exosome treatment (n = 3). (F) Intracellular and extracellular lactate levels in PSCs after exosome incubation (n = 3). (G) ALDOA enzyme activity in PSCs treated with tumor cell-derived exosomes (n = 3). (H) Western blot analysis of α-SMA, VIM, FAP, and GAPDH in PSCs incubated with exosomes under low glucose, 2-DG, or ALDOA knockdown conditions (n = 3). (I) ELISA of IL-6, MCP-1, and FN in the supernatant of PSCs treated with exosomes under low glucose, 2-DG, or ALDOA knockdown (n = 3). ..
Western Blot:Article Title: Engineering exosomes with iRGD for targeted RNAi therapy against pancreatic cancer mediated by long non-coding RNA PLBD1-AS1.
Article Snippet: .. These data suggest that exosomes can effectively deliver siPLBD1-AS1 into target cells and exert biological effects, with iRGD further improving cellular uptake and enhancing their anticancer activity. analysis of α-SMA, VIM, FAP, and β-actin in PSCs after treatment with tumor cell-derived exosomes (n = 3). (C) ELISA of IL-6, MCP-1, and FN levels in the supernatant of PSCs incubated with tumor cell-derived exosomes (n = 3). (E) ECAR and OCR measurements of PSCs following exosome treatment (n = 3). (F) Intracellular and extracellular lactate levels in PSCs after exosome incubation (n = 3). (G) ALDOA enzyme activity in PSCs treated with tumor cell-derived exosomes (n = 3). (H) Western blot analysis of α-SMA, VIM, FAP, and GAPDH in PSCs incubated with exosomes under low glucose, 2-DG, or ALDOA knockdown conditions (n = 3). (I) ELISA of IL-6, MCP-1, and FN in the supernatant of PSCs treated with exosomes under low glucose, 2-DG, or ALDOA knockdown (n = 3). ..
Knockdown:Article Title: Engineering exosomes with iRGD for targeted RNAi therapy against pancreatic cancer mediated by long non-coding RNA PLBD1-AS1.
Article Snippet: .. These data suggest that exosomes can effectively deliver siPLBD1-AS1 into target cells and exert biological effects, with iRGD further improving cellular uptake and enhancing their anticancer activity. analysis of α-SMA, VIM, FAP, and β-actin in PSCs after treatment with tumor cell-derived exosomes (n = 3). (C) ELISA of IL-6, MCP-1, and FN levels in the supernatant of PSCs incubated with tumor cell-derived exosomes (n = 3). (E) ECAR and OCR measurements of PSCs following exosome treatment (n = 3). (F) Intracellular and extracellular lactate levels in PSCs after exosome incubation (n = 3). (G) ALDOA enzyme activity in PSCs treated with tumor cell-derived exosomes (n = 3). (H) Western blot analysis of α-SMA, VIM, FAP, and GAPDH in PSCs incubated with exosomes under low glucose, 2-DG, or ALDOA knockdown conditions (n = 3). (I) ELISA of IL-6, MCP-1, and FN in the supernatant of PSCs treated with exosomes under low glucose, 2-DG, or ALDOA knockdown (n = 3). ..
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